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. 2017 Jan 27;8:14182. doi: 10.1038/ncomms14182

Figure 4. Proliferation and apoptosis assay of spermatogonia.

Figure 4

(a) Immunohistochemical assay of PLZF in control and Bcas2F/−;Vasa-Cre testes at P12. The DNA was stained with hematoxylin. Scale bar, 25 μm. (b) The number of PLZF+ cells per seminiferous tubules in control and Bcas2F/−;Vasa-Cre testes at P12. At least 500 tubules were counted from at least 5 different mice. Error bars represent s.e.m. (c) IF staining of the mitosis marker Ki67 in PLZF positive cells of control and Bcas2F/−;Vasa-Cre testes at P12. The DNA was stained with Hoechst 33342. Scale bar, 20 μm. Arrowheads indicate the representative Ki67+ PLZF+ cells. (d) The ratio of Ki67+ PLZF+ positive cells in PLZF+ cells of control and Bcas2F/−;Vasa-Cre testes at P12 (in %). At least 300 tubules were counted from at least 5 different mice. Error bars represent s.e.m. (e) IF staining of the apoptosis marker cleaved caspase 3 (CAP3) in PLZF+ cells of control and Bcas2F/−;Vasa-Cre testes at P12. The DNA was stained with Hoechst 33342. Scale bar, 50 μm. (f) The number of CAP3+ PLZF+ cells per seminiferous tubules of control and Bcas2F/−;Vasa-Cre testes at P12 (in %). At least 150 tubules were counted from 3 different mice. Error bars represent s.e.m.