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. 2017 Jan 27;8:14237. doi: 10.1038/ncomms14237

Figure 5. FLP-7 is required for OA-induced fat loss.

Figure 5

(a) Vehicle- or OA-treated wild-type and flp-7 mutant animals were fixed and stained with oil Red O. Fat content was quantified for each genotype and is expressed as a percentage of vehicle-treated wild-type animals±s.e.m. (lower panels; n=11-24). ***P<0.001 by two-way ANOVA. (b,c) Representative images for vehicle- and OA-treated wild-type, unc-31, and tph-1 animals bearing integrated FLP-7mCherry and CLM::GFP transgenes. Left columns, GFP expression in coelomocytes; centre panels, secreted FLP-7mCherry uptake in coelomocytes; right panels (merge). Scale bar, 10 μm. (d) The intensity of FLP-7mCherry fluorescence was quantified and normalized to the area of the CLM::GFP. Data are expressed as a percentage of the normalized FLP-7mCherry fluorescence intensity of vehicle-treated wild-type animals±s.e.m. (lower panels; n=8–27). Within group analyses is represented by **P<0.01; (a) P<0.05 across the vehicle treatment groups (black bars); (b) P<0.001 across the OA treatment groups (grey bars) and NS, not significant; two-way ANOVA used for the statistical comparisons.