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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Mol Cancer Res. 2016 Nov 18;15(2):117–127. doi: 10.1158/1541-7786.MCR-16-0281-T

Figure 7.

Figure 7

The ratio of OTUD6B-1/OTUD6B-2 expression is reduced in malignant NSCLC cells and tissue compared to their normal counterparts. A, measurements in NSCLC cell lines and primary airway epithelial cells; qPCR results. The expression has been normalized for three internal controls (specified). The two-way ANOVA results are as follows: the ratio normal/cancer affects the results (p=0.0351); the ratio normal/cancer does not have the same effect at all values of normalization gene (p>0.99); the normalization gene does not affect the results (p>0.99). B, immunoblots for the indicated OTUD6B isoform in the indicated primary cell culture and NSCLC cell lines. Staining the nitrocellulose membrane with Ponceau-S after ECL development assessed equal loading. BE-1, 4F0439; BE-2, 4F0624 (primary bronchial epithelial cells, Cambrex). C, summary of the RT-qPCR results obtained in lungs and NSCLC specimens.