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. Author manuscript; available in PMC: 2017 Nov 15.
Published in final edited form as: Cancer Res. 2016 Sep 20;76(22):6555–6567. doi: 10.1158/0008-5472.CAN-16-0559

Figure 4. Jagged1/Notch1 signaling pathway acts downstream of BRD4 to regulate breast cancer cell migration and invasion.

Figure 4

(A) Representative images from immunofluorescence experiments showing the cellular NICD1 distribution. NICD1 is stained in green and nuclei in blue, with DAPI in merged images (lower panels). Scale bar: 50 μm.

(B) NICD1 nuclear score (for calculation details, see Experimental Procedures section). One dot represents a measurement of one cell. Bars show means ± SEM of three independent experiments. Statistical analyses were performed by using one-way ANOVA.

(C-D) MDA-MB-231 were transfected with the indicated siRNAs (scramble, siBRD4: 50 nM; siJagged1: 20 nM) for 72h prior to conduct migration (C) and invasion (D) assays. Bars show means ± SEM of three independent experiments. Statistical analyses were performed by using one-way ANOVA.

(E-F) MDA-MB-231 were transfected with the indicated siRNAs (scramble, siBRD4: 50 nM; siJagged1: 20 nM) for 72h and plasmids for 24h prior to conduct migration (E) and invasion (F) assays. Bars show means ± SEM of three independent experiments. Statistical analyses were performed by using one-way ANOVA.

The following symbols were used to indicate significant differences: ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001.