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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Cancer Res. 2016 Nov 29;77(3):753–765. doi: 10.1158/0008-5472.CAN-16-0455

Figure 3.

Figure 3

NEK6 is overexpressed in several prostate cancer cell lines compared to immortalized (RWPE, LH) and transformed (LHSR-AR) prostate epithelial cells. A. Left: Expression of NEK6 and AR in prostate cell lines with Hsp90 as loading control; Right: NEK6 expression in LHSR-AR cells (lane 1), LHSR-AR cells overexpressing NEK6 (lane 2), and in VCaP and LNCaP cells. B. Expression of NEK6 in CL-1, PC-3, and DU145 cells with doxycycline inducible expression of 2 shRNAs targeting NEK6 or a control targeting lacZ in the presence or absence of doxycycline (CL-1 and PC-3) or with doxycycline in the presence or absence of growth factor stimulation (DU145). C. Proliferation curves of cells cultured in the presence of doxycycline with cells in 60 mm plates, split and replated every 2–3 d as indicated. The average of 3 replicates with error bars is shown.