Immunohistochemical staining of α3 integrin (A&B), TAZ
(C&D), and YAP (E&F) on adjacent sections
of a prostate cancer tissue microarray. B, D, and F show enlarged views of the
indicated fields in A, C, and E (b, benign gland; Ca, prostate carcinoma).
Arrowheads indicate nuclear TAZ and YAP staining in prostate carcinoma.
(G) Mean staining scores for α3 integrin, TAZ, and YAP
on a 40 case microarray of human prostate cancer and matched normal tissues.
Each case was represented by 4 cores of cancer and 4 cores of normal tissue.
Each point represents the average score for each patient sample, across the 4
cores. The expression of α3 integrin was reduced, while nuclear
expression of TAZ and YAP was increased in tumor versus normal glands
*P<0.0001, paired t test. For scoring TAZ and YAP in normal glands,
only luminal cells were considered.