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. 2004 Dec;24(23):10328–10339. doi: 10.1128/MCB.24.23.10328-10339.2004

FIG. 2.

FIG. 2.

Caspase inhibitor prevents p40 MET generation. (A) MDCK epithelial cells were treated for 12 h with either TNF-α (30 ng/ml)-cycloheximide (Chx; 10 μg/ml) or anisomycin (Ani; 50 μM) in the presence or absence of zVAD-FMK (zVAD; 20 μM) or left untreated (−). For each condition, the same amount of protein was resolved by SDS-10% PAGE and analyzed by Western blotting using an anti-MET antibody directed against its kinase domain. The filter was stripped and reprobed sequentially using an anti-cleaved caspase 3 antibody and an anti-Erk2 antibody to assess loading. (B) NMUMG epithelial cells were treated as for panel A. Protein extracts were analyzed by Western blotting using antibody directed against the C-terminal tail of mouse MET. The filter was stripped and reprobed using an anti-Erk2 antibody. The same extracts were analyzed by Western blotting using an anti-cleaved caspase 3 antibody. The arrowheads indicate the positions of the MET precursor p170, MET subunit p140, and MET p40 fragment.