FIG. 8.

HGF/SF stimulation of the MET receptor does not favor p40 MET generation. MDCK cells were pretreated for 1 h with HGF/SF at the concentration indicated or not pretreated (0) and then were treated for 8 h with anisomycin (Ani.; 50 μM) (+) or left untreated (−). For each condition, caspase 3 activity was determined from the same amount of proteins (n = 3; plus standard deviation). Caspase activity is expressed as pmol/min of degraded substrate. A representative extract was resolved by SDS-10% PAGE and analyzed by Western blotting using an anti-MET antibody directed against the kinase domain. The filter was stripped and reprobed sequentially using an anti-active caspase 3 antibody and an anti-Erk2 antibody.