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. 2016 Dec 5;187(3):353–368. doi: 10.1111/cei.12889

Figure 1.

Figure 1

Effects of apoptotic blebs and freeze–thaw (FT) cellular lysate on the maturation of bone marrow‐derived dendritic cells (BMDCs) derived from NZB × NZW (F1) (NZB/W F1) and BALB/c mice. Immature BMDCs were incubated with apoptotic blebs or FT for 2 days and cells were assessed concurrently for CD11c and the indicated markers by flow cytometry. Cytokines in supernatants were quantified by enzyme‐linked immunosorbent assay (ELISA). (a) Fold change (over the medium control) of CD11c+ BMDCs expressing major histocompatibility complex (MHC)‐I, MHC‐II, CD80, CD83, CD86 and CD40 upon incubation with apoptotic blebs or FT cellular lysate (*P < 0·05; **P < 0·01; ***P < 0·001: apoptotic bleb‐treated NZB/W F1 BMDCs versus apoptotic bleb‐treated BALB/c BMDCs; # P < 0·05, ## P < 0·01, ### P < 0·001: apoptotic bleb‐treated NZB/W F1 BMDCs versus FT cellular lysate‐treated NZB/W F1 BMDCs). (b) Cytokine secretion from BMDCs derived from NZB/W F1 and BALB/c mice incubated with apoptotic blebs or FT cellular lysate (*P < 0·05; **P < 0·01). Data represent mean ± standard error of the mean (s.e.m.) of four experiments.