TABLE 1.
Yeast strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant genotype, description, or sequence | Source | ||
|---|---|---|---|---|
| Parental strains | ||||
| YJB195 | MATaade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | W303a | ||
| YJB209 | MATα ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | W303α | ||
| YJB1463 | MATaVR-ADE2-TEL | S. Enomoto | ||
| YJB1801 | MATacac2::TRP1 | S. Enomoto | ||
| YJB1804 | MATacac1::LEU2 cac2::TRP1 | S. Enomoto | ||
| YJB1862 | MATacac1::LEU2 cac3::hisG | S. Enomoto | ||
| YJB2704 | MATanap1::LEU2 | S. Enomoto | ||
| YJB4713 | MATahir3::HIS3 | S. Enomoto | ||
| YJB6277 | MATaasf1::his5+ | S. Enomoto | ||
| YJB6165 | MATacac1::LEU2 | S. Enomoto | ||
| YJB7649 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 | Spore colony from YJB7344 × YJB1463 | ||
| YJB8349 | MATakap123::HIS3 | YJB195 transformed with kap123::HIS3 | ||
| YJB8420 | MATacac1::LEU2 | This study | ||
| Histone plasmid strains | ||||
| YJB344 | Δ(hht1-hhf1) Δ(hht2-hhf2)/p632 (H4-WT) | 41 | ||
| YJB347 | Δ(hht1-hhf1) Δ(hht2-hhf2)/p860 (H4-4A) | 41 | ||
| YJB348 | Δ(hht1-hhf1) Δ(hht2-hhf2)/p861 (H4-4R) | 41 | ||
| YJB2318 | Δ(hht1-hhf1) Δ(hht2-hhf2)/p632/p860 | YJB344 transformed with p860 | ||
| YJB2861 | hhf1::HIS3 hhf2::LEU2/p974 (H4-4Q) | 16 | ||
| H4-WT integrated strains | ||||
| YJB7349 | hhf2-hht2::CaURA3 | Spore colony from YJB1463 × YJB7345 | ||
| YJB7351 | HHF1/HHF2 | Spore colony from YJB1463 × YJB7345 | ||
| YJB7418 | HHF1/HHF2 | Spore colony from YJB1463 × YJB7345 | ||
| H4-4A integrated strains | ||||
| YJB7344 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 | YJB7143 transformed with hhf2-hht2::CaURA3 | ||
| YJB7345 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 | YJB7143 transformed with hhf2-hht2::CaURA3 | ||
| YJB7350 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 | YJB7151 transformed with hhf2-hht2::CaURA3 | ||
| YJB7354 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 | Spore colony from YJB1463 × YJB7344 | ||
| H4-4A/WT heterozygote integrated strains | ||||
| YJB7143 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 HHF2 | This study | ||
| YJB7151 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 HHF2 | This study | ||
| YJB7348 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 HHF2 | Spore colony from YJB1463 × YJB7345 | ||
| YJB7355 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 HHF2 | Spore colony from YJB1463 × YJB7345 | ||
| YJB7356 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 HHF2 | Spore colony from YJB1463 × YJB7345 | ||
| YJB8396 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 asf1::his5+ | Spore colony from YJB6277 × YJB7649 | ||
| YJB8398 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 cac1::LEU2 | Spore colony from YJB6165 × YJB7649 | ||
| YJB8400 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 hir3::HIS3 | Spore colony from YJB4713 × YJB7354 | ||
| YJB8402 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 nap1::LEU2 | Spore colony from YJB2704 × YJB7649 | ||
| YJB8405 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 kap123::HIS3 | Spore colony from YJB7649 × YJB8349 | ||
| YJB8817 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 cac2::TRP1 | Spore colony from YJB1801 × YJB7649 | ||
| URA3-TEL strains | ||||
| YJB8602 | MATaVIIL::URA3-TEL | YJB195 transformed with p105 | ||
| YJB8603 | MATacac1Δ VIIL::URA3-TEL | YJB8420 transformed with p105 | ||
| YJB8604 | MATahhf2-4A::KanR-HHT2::hhf1-hht1 VIIL::URA3-TEL | YJB7143 transformed with p105 | ||
| N-terminal histone H4-GFP fusion strains | ||||
| YJB8342 | HHF1/HHF2 p2087 (H4-WT-GFP) | YJB195 transformed with p2087 | ||
| YJB8343 | HHF1/HHF2 p2088 (H4-4A-GFP) | YJB195 transformed with p2088 | ||
| YJB8432 | cac1Δ p2087 (H4-WT-GFP) | YJB8420 transformed with p2087 | ||
| YJB8378 | cac1Δ p2088 (H4-4A-GFP) | YJB8420 transformed with p2088 | ||
| YJB9010 | HHF1/HHF2 p2115 (H4-K8R-GFP) | YJB195 transformed with p2115 | ||
| YJB9011 | HHF1/HHF2 p2116 (H4-K5,12R-GFP) | YJB195 transformed with p2116 | ||
| YJB9012 | HHF1/HHF2 p2021 (H4-4R-GFP) | YJB195 transformed with p2021 | ||
| YJB9013 | cac1Δ cac2Δ p2087 (H4-WT-GFP) | YJB1804 transformed with p2087 | ||
| YJB9014 | cac1Δ cac2Δ p2088 (H4-4A-GFP) | YJB1804 transformed with p2088 | ||
| YJB9015 | cac1Δ cac3Δ p2087 (H4-WT-GFP) | YJB1862 transformed with p2087 | ||
| YJB9016 | cac1Δ cac3Δ p2088 (H4-4A-GFP) | YJB1862 transformed with p2088?xpp Tr>Strain, plasmid, or primer | Relevant genotype, description, or sequence | Source |
| Chromatin immunoprecipitation strains | ||||
| YJB7650 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 hhf2-hht2::CaURA3 sir3::SIR3-HA-TRP1 | YJB7354 transformed with sir3::SIR3-HA-TRP1 | ||
| YJB7651 | MATα hhf2-hht2::CaURA3 sir3::SIR3-HA-TRP1 | YJB7349 transformed with sir3::SIR3-HA-TRP1 | ||
| YJB7902 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 sir3::SIR3-HA-TRP1 | YJB7143 transformed with sir3::SIR3-HA-TRP1 | ||
| YJB8651 | MATα hhf2-4A::KanR-HHT2::hhf1-hht1 p1307 (Sir3-HA) | YJB7143 transformed with p1307 | ||
| Plasmid | ||||
| p105 | VIIL-URA3-TEL | 12 | ||
| p632 | CEN4 ARS1 LYS2 HHT2-HHF2 | 42 | ||
| p860 | CEN4 ARS1 URA3 HHT2-hhf2-4A | 42 | ||
| p920 | CEN6 ARS4 LEU2 HHT1-hhf1-K5,12R | 34 | ||
| p925 | CEN6 ARS4 LEU2 HHT1-hhf1-K8R | S. Enomoto | ||
| p1307 | 2μm TRP1 SIR3-HA | 17 | ||
| p1540 | CEN4 ARS1 URA3 HHT2-hhf2-4R | 42 | ||
| p2077 | CEN6 ARS4 HIS3 MET3::HHF2(aa 1-41) | This study | ||
| p2078 | CEN6 ARS4 HIS3 MET3::hhf2-4A(aa 1-41) | This study | ||
| p2087 | CEN6 ARS4 HIS3 TRP1 MET3::HHF2-GFP | This study | ||
| p2088 | CEN6 ARS4 HIS3 TRP1 MET3::hhf2-4A-GFP | This study | ||
| p2113 | CEN6 ARS4 HIS3 MET3::hhf2-K5,12R(aa 1-41) | This study | ||
| p2114 | CEN6 ARS4 HIS3 MET3::hhf2-K8R(aa 1-41) | This study | ||
| p2117 | CEN6 ARS4 HIS3 MET3::hhf2-4R(aa 1-41) | This study | ||
| p2115 | CEN6 ARS4 HIS3 TRP1 MET3::hhf2-K8R-GFP | This study | ||
| p2116 | CEN6 ARS4 HIS3 TRP1 MET3::hhf2-K5,12R-GFP | This study | ||
| p2121 | CEN6 ARS4 HIS3 TRP1 MET3::hhf2-4R-GFP | This study | ||
| Primer | ||||
| 340 | ATGGAAGCTTCACATTTGC | |||
| 404 | CATGATGTATACATTGTGTGAGATATAGTTGTATTCCA ATTTGTGTCC | |||
| 684 | ATCCCCTGCAATAGTAGCGATAGTAAAAAGAGGCGCA TACATCCTACGCCAGTCGATTTGCGGATCCCCGGGT TAATTAA | |||
| 685 | AGCACACAATGGAAAATATCTTCGCTGGCAAACTAGA TTAGGCATTCTTATGTACCGCATGAATTCGAGCTCG TTTAAAC | |||
| 885 | GCCCAGATCTTCCCGCTTTATATTCATGATCTTTC | |||
| 886 | GCCCAGATCTTTAACCACCGAAACCATATAAGG | |||
| 943 | ATATTAGGATGAGGCGGTGA | |||
| 948 | TGTTTTTGTTCGTTTTTTACTAAAACTGATGACAATCA AATATTCATGATCTTTCACCT | |||
| 978 | TAACACGCTTAGCGTGAATAGCAGCCAGATTAGTGTC TTCCGATAGGCCACTAGTGGATCTG | |||
| 979 | TGTAAGTAACAGAGTCCCTGATGACGGATTCCAAGAA GGACAGCTGAAGCTTCGTACGC | |||
| 1001 | CTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGT GAATTCGAGCTCGTTTAAAC | |||
| 1002 | CACAGAGTAAATTCCCAAAT | |||
| 1293 | AACTGAGGGACGAAAAACACTTTTTTAGTATCAAGTA GTATAATGCGGATCCCCGGGTTAATTAA | |||
| 1294 | GTTTATCGAAACAGACGAGAATAAAAAATGGTTTTAA AAAAATCAGAATTCGAGCTCGTTTAAAC | |||
| 1358 | GTTGTGATATCGCGTTTATTAAAATGTCCGGTAGAGGT AAAGGTGGT | |||
| 1359 | GTTGTGATATCGCGTTTATTAAAATGTCCGGTAGAGGT GCCGGTGGT | |||
| 1360 | CGCGGATCCTAATCTTCTGATAGCTGGCTTAGT | |||
| 1427 | TAACATTCAAGGTATCACTAAGCCAGCTATCAGAAGAA TACGGATCCCCGGGTTAATTAA | |||
| 1428 | CTCACTATAGGGCGAATTGGAGCTCCACCGCGGTGGCG GCGAATTCGAGCTCGTTTAAAC | |||
| 1694 | GTTGTGATATCGCGTTTATTAAAATGTCCGGTAGAGGTC GGGGTGGT |