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. 2017 Jan 30;8:14076. doi: 10.1038/ncomms14076

Figure 7. The ELLBow-binding site of ELL2 is important for HIV-1 long terminal repeat (LTR) transcription.

Figure 7

(a,b) Luciferase activities were measured and analysed in extracts of cells transfected in triplicate with the HIV-1 LTR-luciferase construct together with the combinations of plasmids expressing wild-type and mutant ELL2-HA and FLAG-AFF4 as indicated. Each of the ELL2 and AFF4 plasmids was transfected at 1 μg per well. The activities in the control groups were set to 1. The error bars represent mean ±s.d. from triplicate wells. An aliquot of each cell extract was examined by immunoblotting for presence of the various proteins labelled on the left. (c) Jurkat 2D10 cells were nucleofected in triplicates with plasmids expressing AFF1 (top) or AFF4 (bottom) alone or in combination with ELL2wt or its mutants as indicated. 48 h post nucleofection, the percentages of GFP+ cells were measured by flow cytometry and plotted. The error bars represent mean ±s.d.'s. The levels of the indicated proteins in nucleofected cells were determined by immunoblotting. Uncropped versions of the blots are shown in Supplementary Fig. 6.