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. 2017 Jan 30;8:14286. doi: 10.1038/ncomms14286

Figure 1. In vitro orthogonal reactivity of the xUB-xUba6 and xUB-xUba1 pairs.

Figure 1

(a) xUB and wt UB activation by the xE1 and wt E1 enzymes in the ATP-PPi exchange assay. Data are shown as means±s.e.m. (n=3). (b) Formation of UB∼E1 and UB∼E2 thioester conjugates with xUB and xUba1. UBE2D2/UbcH5b was used as the E2. Cross-reactivity between xUB and wtUba1, and between wtUB and xUba1 was not detected. (c) xUba1 can transfer xUB to UbcH5b and CHIP. CHIP autoubiquitination by wt UB and wt Uba1 were used as a positive control. (d) Same as (b) with the xUB-xUba6 pair. (e) Same as (c) with the xUB-xUba6 pair.