Skip to main content
. 2017 Jan 30;8:14286. doi: 10.1038/ncomms14286

Figure 3. Uba6 is required for polyubiquitination of the RNA binding protein CUGBP1 and the actin-binding protein ezrin.

Figure 3

(a) CUGBP1 and ezrin are polyubiquitinated. HEK293 cells were transfected with polyhistidine-HA-tagged ubiquitin (UB), and treated with 10 μM MG132 for 90 min. The indicated proteins were immunoprecipitated (IP), followed by immunoblotting (IB) for the polyhistidine tag. IgG, control immunoprecipitation with normal rabbit or mouse IgG. The right panels indicate immunoblotting with total cell lysates for the indicated proteins. (b) Silencing UBA6 results in decreased polyubiquitination of CUGBP1 and ezrin, while silencing UBA1 increases polyubiquitination of these proteins. HEK293 cells were lentivirally transduced with anti-UBA6, anti-UBA1 or control (Ctrl) shRNA. Following drug selection, stable cell populations were immunoprecipitated followed by immunoblotting, as indicated. The asterisks indicate background bands from IgG.