Figure 5. Uba6 is required for the regulation of subcellular localization of ezrin and formation of epithelial acini in human nontransformed mammary epithelial MCF-10A cells.

(a) Immunofluorescence microscopy for ezrin, F-actin (via phalloidin) and chromosomal DNA (via Hoechst 33342) in cell spheroids formed in 3D culture at day 14. Representative pictures are shown from three groups such as control, shUBA6 and shUBA6 plus FLAG-Uba6 cDNA (OE). (b) Quantification of spheroids without lumen in day 14 culture. (c) The average size of spheroids in each experimental group relative to the control group. The mean acinar structure area in each group was quantified using ImageJ software (see Supplementary Fig. 7). (d) Quantification of spheroids with mislocalized ezrin. **P<0.01; *P<0.05. Data are shown as means+s.e.m. from three biological replicates.