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. 2017 Jan 30;8:14286. doi: 10.1038/ncomms14286

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Human non-transformed mammary epithelial MCF-10A cells stably expressing the indicated shRNAs were generated by lentiviral transduction and drug selection, and examined for epithelial morphogenesis by 3D culture. (a) Immunofluorescence microscopy for ezrin and chromosomal DNA (via Hoechst 33342) in cell spheroids formed in 3D culture. (b) Quantification of spheroids with lumen formation. Data are shown as means+s.e.m. from three biological replicates.