Figure 7. Adhesion signaling via vinculin is required for an effective cellular clock.

(a) Primary MECs expressing the GFP-sh-vinculin (outlined in yellow) have almost undetectable levels of vinculin. In contrast, cells not expressing GFP (green) have strong vinculin expression (shown by IF, red). Blue, DAPI. N=3 animals, scale bar=20 μm. (b) Western blotting to confirm knockdown of vinculin in GFP-sorted MEC cells. Note: as a control, the levels of ERK were not affected by sh-vinculin. (c) Representative PER2::Luc traces from GFP-sorted MEC cells expressing lentiviral shRNAs against vinculin (red) or empty vector control (black). N=4 animals.