FIGURE 1.

Alloprimed T cells from transplanted mice induce vascular endothelial cell proliferation. A, VECs were cultured alone (medium), or with allogeneic or syngeneic T cells collected from naive, LR, or HR transplanted mice. After 24 hours, the proliferation of VECs was assessed using the BrdU incorporation assay. B, Purified, allogeneic or syngeneic T cells were cocultured with APCs for 72 hours. BrdU reagent was added to each well 16 hours before collecting the cells, and T cell proliferation was measured. Each experiment has been performed at least 2 times, with 4 mice per group per experiment.