TABLE 1.

Target genes and silencing efficiencies of the pH1RNAi vectors

Target gene	siRNA target sequence (N19)	Nucleotide position from ATG	Mean target gene expression ± SD (% of control level)
Laminin αI	TACCAGAAGGGAGATTGAC	1968	31.2 ± 6.2
Hnf1β	GCAQTCAGGATCAGCTGCT	590	31.5 ± 4.2
Gata4	GGCAGAGAGTGTGTCAATT	637	42.1 ± 1.9
Gata6	TGCGTTGCAGCAATCAGTG	1993	18.5 ± 0.9
Sox7	GATCTACCCTGGTTCGGAA	1988	33.7 ± 3.2
Cited1	GGATCGCAAGGCAGTGACT	111	32.9 ± 3.3
Cited2	GTGCTTATGTCCTTAGTGA	508	15.9 ± 4.7
Coup-tf2a	GCTGTACAGAGAGGCAGGA	442	46.1 ± 2.1
	GTGGAGAAGCTCAAGGCAC	895
	GAGTACGTTAGGAGCCAGT	1039
	GGATGTTACAAGTTTGCTA	1343
Epas1	GCCAGAACTTCGATGAACC	1253	36.2 ± 6.7
Atf3	GAGCTGAGATTCGCCATCC	130	32.2 ± 1.1
Sox17	GTCTTGGAAGGCGTTGACC	312	68.8 ± 0.62
Foxa2/Hnf3β	GACATACCGACGCAGCTAC	447	76.0 ± 4.4
Coup-tf1	CTTACACATGCCGTGCCAA	353	88.3 ± 25.5

^aA mixture of four vectors harboring different N19 sequences was used for transfection.