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. 2004 Dec;24(23):10193–10207. doi: 10.1128/MCB.24.23.10193-10207.2004

FIG. 6.

FIG. 6.

RNA Pol II ChIP. Formaldehyde cross-linked chromatin was isolated from forward and reverse orientation cell lines that were left uninduced, treated with doxycycline (6 h), or treated with doxycycline (6 h) and α-amanitin (1 h). Chromatin was immunoprecipitated with antibodies against RNA Pol II or normal rabbit serum. Q-PCR of the immunoprecipitated DNA was carried out at the STSs indicated. The data are represented as the abundance of Pol II-bound DNA in the induced or induced plus α-amanitin-treated cells relative to that of the RNA Pol II bound DNA in uninduced cells. Error bars indicate standard deviations.