Table 2.
Virulence marker | Locus description | Primers | Frag. (bp) | Tm (°C) | References |
---|---|---|---|---|---|
EAEC VIRULENCE GENES | |||||
aatA | Dispersin transporter (earlier pCVD432) | CTGGCGAAAGACTGTATCAT | 630 | 60 | Pereira et al., 2007 |
CCATGTATAGAAATCCGCTGTT | |||||
aggR | Transcription activator | CTAATTGTACAATCGATGTA | 324 | 50 | Pereira et al., 2010 |
CTGAAGTAATTCTTGAAT | |||||
aapA | Dispersin | CTTTTCTGGCATCTTGGGT | 328 | 56 | This study |
TTATTTAACCCATTCGGTTAGAGC | |||||
aggA | Aggregative adherence fimbria (AAF-I) | GCTAACGCTGCGTTAGAAAGACC | 421 | 60 | Pereira et al., 2007 |
GGAGTATCATTCTATATTCGCC | |||||
aafA | AAF-II | GACAACCGCAACGCTGCGCTG | 233 | 50 | Pereira et al., 2007 |
GATAGCCGGTGTAATTGAGCC | |||||
agg-3A | AAF-III | GTATCATTGCGAGTCTGGTATTCAG | 462 | 56 | Bernier et al., 2002 |
GGGCTGTTATAGAGTAACTTCCAG | 58 | ||||
hdaB-A | AAF-IV | CTGTAGGACGTAGGTAATGAAACTG | 993 | This study | |
AAACTCCAGGCGTTAACGTCTG | |||||
agg-5A | AAF-V | AGCAGCAACAGCAAATCC | 342 | 56 | This study |
CCGTAACCACTTCCTCGT | |||||
pet | Plasmid-encoded toxin | CCGCAAATGGAGCTGCAAC | 1133 | 60 | Pereira et al., 2007 |
CGAGTTTTCCGCCGTTTTC | |||||
pic | Mucinase | TTCAGCGGAAAGACGAA | 500 | 60 | Pereira et al., 2007 |
TCTGCGCATTCATACCA | |||||
UPEC VIRULENCE GENES | |||||
fyuA | Yersiniabactin siderophore receptor | TGAGTGGGAAATACACCACC | 715 | 54 | This study |
TTACCCGCATTGCTTAATGTC | |||||
yfcV | YfC fimbria | ATCCGTGTTGGCTGGC | 280 | 54 | This study |
GGTCATGGGCGCAGTT | |||||
chuA | Heme receptor | TAACTGTCATAGCGGGTTCC | 439 | 55 | This study |
AGTCTCTGAGCGGTTTAGTG | |||||
vat | Vacuolating autotransporter toxin | CAGAACATTTGCTCCCTTGT | 1102 | 53 | This study |
ACACGTTCAGGATTCAGT | |||||
papC | Pyelonephritis-associated pilus | GACGGCTGTACTGCAGGGTGTGGCG | 328 | 60 | Daigle et al., 1994 |
ATATCCTTTCTGCAGGGATGCAATA | |||||
focA | Fimbria F1C (focA-sfaD) | GAAAGTAGATGGAGCTAAAAGCAAT | 496 | 54 | This study |
CATGACATGCCAGTGGTTTC | |||||
sfa | Fimbria S | CTCCGGAGAACTGGGTGCATCTTAC | 407 | 60 | Daigle et al., 1994 |
CGGAGGAGTAATTACAAACCTGGCA | |||||
cnf | Cytotoxic necrotizing factor | GTGAAGCTCAACGAGACTAT | 826 | 53 | This study |
TCAGTAGCTCCTCTCATCAA | |||||
GENERAL E. COLI ADHESINS | |||||
csgA | Curli fimbria | CTCTGACTTGACTATTACC | 199 | 50 | This study |
AGATGCAGTCTGGTCAAC | |||||
ag43 | Self-recognizing protein (adhesin) | CGATCGATAAGCTAATAATAACC | 552 | 55 | This study |
GAAGACCACCACTGGTGACA | |||||
pilS | Plasmid-encoded type IV pili | ATGAGCGTCATAACCTGTTC | 532 | 58 | Dudley et al., 2006 |