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. 2017 Feb 3;8:23. doi: 10.3389/fphar.2017.00023

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Copyright © 2017 Rouleau, Audet-Delage, Desjardins, Rouleau, Girard-Bock and Guillemette.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of selected protein interactions by immunoprecipitation and immunofluorescence in a UGT negative kidney cell model. (A) Immunoprecipitation (IP) of UGT1A9, with purified anti-UGT1A antibodies, was conducted in HEK293-UGT1A9_myc/his transiently transfected with the indicated protein partner. UGT1A9 was immunodetected with anti-myc, whereas protein partners were detected with anti-tag antibodies as specified below immunoblots. Control IPs were conducted with normal rabbit immunoglobulins (IgG). Lysates (IP input) are shown as references. Protein bands denoted by the asterisk are the rabbit IgGs used in IPs. (B) Co-localization of UGT1A9 and the protein partners ACOT8 and SH3KBP1/CIN85 assessed by immunofluorescence in HEK293-UGT1A9_myc/his transiently expressing specified partners. Confocal microscope images are representative of three independent experiments. Partial co-localization is detected by yellow labeling in merged images. Insets present enlargements of boxed regions in merged images. Bar = 20 μm.