FIG. 3.

Yeast two-hybrid assay showing that a mutation in SPP2 can restore the interaction with mutant prp2. (A) Retransformation of strain CG1945 carrying the indicated pGBD-derived plasmid with the parental GAD-SPP2 or the isolated GAD-SPP2^L109V plasmid. The transformants were streaked and grown on a plate omitting histidine at 30°C for 4 days. (B) The GAD-SPP2^L109V plasmid was recreated via site-directed mutagenesis and transformed into CG1945 strains containing GBD derivatives. The transformants were grown, diluted as indicated, and spotted onto a plate containing histidine (+His) and a plate without histidine (−His). WT, wild type; DC/NY, D845N/C846Y; WL/AA, W854A/L855A.