Figure 2. Comparison of Pink1 DNA Targeting by Injection of Cas9 mRNA and Cas9 Protein.

Identification of Pink1 DNA mutations by T7E1 assay in monkey embryos injected with WT-Cas9 or Ubi-Cas9 mRNA (A) or purified WT-Cas9 protein (B). Representative DNA gel images showing PCR results of single cells from different embryos. Arrow indicates DNA mutation in a single cell from 8-cell embryos. PC is a positive control from embryos injected with Cas9 mRNA and gRNA for Pink1 targeting. M: Molecular markers. (C) Comparison of targeting rates of injected Cas9 protein, WT-Cas9 mRNA, and Ubi-Cas9 mRNA in monkey embryos. The numbers of embryos with targeted gene mutations or embryos containing mutations in all cells are presented. Embryos at the 4- and 8-cell stages were used, and data were obtained from six experiments.