TABLE 2.
Plasmids used in this studya
| Plasmid | Description | Sourceb |
|---|---|---|
| pAE100 | pRS316 ADH1P-GAL4(1-147)-SIR1 | J. Rine |
| pAE108 | pRH98-1 GPDP-GAL4(1-147)-ORC2 | J. Rine |
| pAE109 | pRH98-1 GPDP-GAL4(1-147)-ORC5 | J. Rine |
| pAE405 | pRS316 ORC1 | |
| pAE408 | pTT64 GAL4(1-147)-ORC1(5-267) | R. Sternglanz |
| pAE516 | pRH98-1 GPDP-GAL4(1-147)-ORC6 | |
| pAE580 | pRS316 SIR3-GFP | D. Shore |
| pAE595 | pRH98-1 GPDP-GAL4(1-147)-ORC3 | |
| pAE597 | pRH98-1 GPDP-GAL4(1-147)-ORC4 | |
| pAE877 | pRS306 ORC1(1-250)-TAP | |
| pAE989 | pRS306 orc1-A2P(1-250)-TAP | |
| pAE990 | pRS306 orc1-A2V(1-250)-TAP | |
| pAE1007 | pRS306 SIR3(1-235)-TAP | |
| pAE1023 | pRS306 sir3-A2T(1-235)-TAP | |
| pAE1026 | pRS306 sir3-A2P(1-235)-TAP | |
| pAE1027 | YIplac204 sir3-A2P(1-502) |
a
Cloning details are available from the authors upon request.
b
Unless indicated otherwise, plasmids were constructed during the course of this study or were from the laboratory plasmid collection.