FIG. 4.
RAD53 and genes required for its activation are required for viability of clb5Δ cells. Diploid strains OAy967, OAy931, DGy534, OAy981, and OAy969 (panels A, B, C, D, and E, respectively) were induced to sporulate at 23°C. Germination was carried out at 30°C, except for panel E, which was carried out at 23°C. The relevant genotypes are indicated in the panels below as follows: WT, wild type; r53, rad53Δ; c5, clb5Δ; m1, mec1Δ; t1, tel1Δ; r9, rad9Δ; mAQ, mrc1AQ; and mr, mrc1Δ. The genotypes of inviable spores were inferred by assuming 2:2 segregation of all genetic loci within each tetrad.