TABLE 1.
Yeast strains used in this study
| Straina | Genotype | Source or reference |
|---|---|---|
| OAy470 | MATaade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 bar1Δ::hisG | 3 |
| DGy159 | sml1Δ::HIS3 mec1Δ::TRP1 bar1Δ::LEU2 | 2 |
| FHy20 | rad53Δ::RAD53-HA3 (TRP1) | 2 |
| JAy30 | clb5Δ::kanMx | 2 |
| DGy138 | clb6Δ::kanMx rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy147 | clb5Δ::HIS5 rad53Δ::RAD53-HA3 (TRP1) clb6Δ::LEU2 | This study |
| DGy149 | sml1Δ::HIS3 clb5Δ::kanMx | This study |
| DGy155 | sml1Δ::HIS3 rad53Δ::kanMx BAR1 | This study |
| DGy160 | sml1Δ::HIS3 bar1Δ::LEU2 | This study |
| DGy162 | clb5Δ::kanMx sml1Δ::HIS3 mec1Δ::TRP1 bar1Δ::LEU2 | This study |
| DGy163 | sml1Δ::HIS3 mec1Δ::TRP1 bar1Δ::LEU2 clb5Δ::kanMx rad53Δ::RAD53-HA3 (URA3) | This study |
| DGy183 | rad9Δ::HIS5 | This study |
| DGy190 | rad9Δ::HIS5 clb5Δ::URA3 | This study |
| DGy207 | clb5Δ::kanMx sml1Δ::HIS3 bar1Δ::LEU2 | This study |
| DGy213 | sml1Δ::HIS3 clb5Δ::kanMx bar1Δ::URA3 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy226 | clb5Δ::URA3 | This study |
| DGy400 | rad9Δ::HIS5 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy410 | rad9Δ::HIS5 clb5Δ::URA3 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy430 | DDC2-GFP (TRP1) | This study |
| DGy431 | clb5Δ::kanMx DDC2-GFP (TRP1) | This study |
| DGy438 | clb5::CLB6 | This study |
| DGy439 | clb5::CLB6 clb6Δ::kanMx | This study |
| DGy448 | clb5::CLB6 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy449 | clb5::CLB6 clb6Δ::kanMx rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy511 | sml1Δ::HIS3 bar1Δ::LEU2 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy516 | mrc1Δ::kanMx::mrc1AQ(LEU2) clb5Δ::HIS5 | This study |
| DGy529 | mrc1Δ::URA3::mrc1AQ(LEU2) bar1Δ::kanMx rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy530 | mrc1Δ::kanMx::mrc1AQ(LEU2) clb5Δ::HIS5 rad53Δ::RAD53-HA3 (URA3) | This study |
| DGy531 | sml1Δ::HIS3 mec1Δ::TRP1 bar1Δ::LEU2 rad53Δ::RAD53-HA3 (TRP1) | This study |
| DGy532 | sml1Δ::HIS3 mrc1Δ::TRP1::mrc1AQ (LEU2) BAR1 | This study |
| DGy534 | DGy532 × OAy980 diploid | This study |
| SSy60 | mrc1Δ::URA3::mrc1AQ (LEU2) bar1Δ::kanMx | This study |
| SSy68 | rrm3Δ::TRP1 BAR1 | This study |
| OAy850 | clb5Δ::HIS5 rad53Δ::RAD53-HA3 (TRP1) | This study |
| OAy874 | MATα sml1Δ::HIS3 mec1Δ::TRP1 | This study |
| OAy931 | OAy874 × DGy207 diploid | This study |
| OAy945 | MATα sml1Δ::HIS3 clb5Δ::URA3 | This study |
| OAy966 | MATα sml1Δ::HIS3 rad53-11 BAR1 | This study |
| OAy967 | DGy155 × OAy945 diploid | This study |
| OAy969 | OAy971 × OAy972 diploid | This study |
| OAy970 | DGy149 × OAy966 diploid | This study |
| OAy971 | MATα sml1Δ::HIS3 clb5Δ::URA3 mec1Δ::TRP1 | This study |
| OAy972 | sml1Δ::HIS3 tel1Δ::kanMx BAR1 | This study |
| OAy977 | sml1Δ::HIS3 mec1Δ::TRP1 BAR1 | This study |
| OAy978 | MATα sml1Δ::HIS3 clb5Δ::URA3 BAR1 | This study |
| OAy980 | MATα sml1Δ::HIS3 clb5Δ::URA3 rad9Δ::kanMx BAR1 | This study |
| OAy981 | OAy977 × OAy980 diploid | This study |
| OAy982 | tel1Δ::kanMx sml1Δ::HIS3 BAR1 | This study |
| OAy983 | MATα mec1Δ::TRP1 sml1Δ::HIS3 BAR1 | This study |
| OAy988 | OAy978 × OAy987 diploid | This study |
| OAy989 | OAy978 × SSy68 diploid | This study |
| OAy990 | sml1Δ::HIS3 mec1Δ::TRP1 tel1Δ::kanMx | This study |
| OAy991 | sml1Δ::HIS3 clb5Δ::URA3 mec1Δ::TRP1 tel1Δ::kanMx | This study |
a
Strains are like OAy470 unless otherwise noted.