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. 2017 Feb 3;7:41775. doi: 10.1038/srep41775

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Mycobacterial lipids were extracted from 3-week culture of planktonic bacteria (A‒D) and pellicle bacteria. (E‒H) of MAH OCU806 (A,E), MAH 104 (B,F), MAH OCU817 (C,G) and MAH 104 R (D,H), and each sample was applied on 2D-TLC. GPLs (I), TDM (J,L) and TMM (K,M) were quantified using image analyser. Data of pellicle bacteria were represented as the ratio of the density to the respective sample of planktonic bacteria. Data were expressed as means ± S.D from three independent experiments. Pkt = planktonic bacteria, Pel = Pellicle bacteria. See also Supplementary Fig. S6 for the mass spectrometry data to confirm the assignments of glycolipids.