FIG. 6.

Loss of Asf1p increases Ddc2p-GFP foci formation. (A) A typical metaphase asf1Δ cell with the Ddc2p-GFP focus indicated by the arrow. A single focus is the site of repair of multiple DNA lesions and the majority of cells with Ddc2p-GFP foci in this analysis contained a single focus. (B) Proportion of asynchronous cells containing Ddc2p-GFP foci in strain JRY006 (WT), JRY007 (asf1Δ), and JRY008 (rad52Δ). (C) Cell cycle distribution of asf1Δ cells containing Ddc2p-GFP foci, as determined by bud morphology, where “none,” “small,” and “large” refer to the bud size.