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. 2004 Dec;186(23):8026–8035. doi: 10.1128/JB.186.23.8026-8035.2004

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Copyright © 2004, American Society for Microbiology

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FIG. 3. — Concentration dependence of RuBP on the binding of CbbR_I and CbbR_II to their cognate promoters. Phosphorimage of gel mobility shift assays are shown with CbbR_I (0.08 nmol) and a cbb_I probe (A) and CbbR_II (0.71 nmol) and a cbb_II probe (B). Lane 1, CbbR with no metabolite added; lane 2, 1 μM RuBP; lane 3, 10 μM RuBP; lane 4, 100 μM RuBP; lane 5, 1.0 mM RuBP; lane 6, probe only. All reactions contained 3.7 μg of poly(dI-dC)::poly(dI-dC) and 15,000 cpm of ³²P-labeled probe. Arrows indicate unbound probe (U) and shifted protein-DNA complexes.