FIG. 5.
Effect of fructose-1,6-bisphosphate on the DNase I-protected region caused by CbbRII binding to the cbbII promoter. The phosphorimage of a DNase I footprint is shown. The probe fragment used spans nucleotides −151 to +46 relative to the cbbF transcription start for the top strand (A) and −151 to +79 on the bottom strand (B) is labeled on the top and bottom strands. Brackets indicate regions of protection, and asterisks indicate DNase I-hypersensitive sites. A control lane that does not contain CbbRII is shown along with a lane containing a standard Maxim-Gilbert A+G sequencing ladder of the probe. The concentration of fructose-1,6-bisphosphate present in each reaction is indicated. Unless otherwise indicated, all reactions contained 3.7 μM CbbRII.