TABLE 2.
β-Galactosidase activities from an adhI-lacZ reporter gene in wild type cells and those lacking combinations of rfdRTS or afdRTS
| Strain | Plasmid | Relevant genotype | Inducerb | β-Galactosidase activity Miller units (SD)a |
|---|---|---|---|---|
| Wild type | None | afdRTS+/rfdRTS+ | None | 26 (7) |
| Wild type | None | afdRTS+/rfdRTS+ | MeOH | 381 (125) |
| Wild type | None | afdRTS+/rfdRTS+ | HCHO | 295 (100) |
| RfdR1 | None | rfdRTS deficient | None | 74 (2) |
| RfdR1 | pRfd4 | rfdRTS+ | None | 31 (7) |
| RfdR1 | pRfdT4 | rfdT deficient | None | 23 (1) |
| RfdR1 | None | rfdRTS deficient | MeOH | 755 (145) |
| RfdS1 | None | rfdS deficient | None | 66 (9) |
| RfdS1 | pRfd4 | rfdRTS+ | None | 33 (2) |
| RfdS1 | None | rfdS deficient | MeOH | 651 (77) |
| AfdR7 | None | afdRTS deficient | None | 17 (5) |
| AfdR7 | None | afdRTS deficient | MeOH | 17 (5) |
| AfdR7 | None | afdRTS deficient | HCHO | 16 (5) |
| AfdR7-5 | None | afdRTS+ | None | 31 (4) |
| AfdR7-5 | None | afdRTS+ | MeOH | 482 (113) |
| AfdR7-5 | None | afdRTS+ | HCHO | 317 (59) |
| AfdS1 | None | afdS deficient | None | 28 (13) |
| AfdS1 | None | afdS deficient | MeOH | 42 (14) |
| AfdS1 | None | afdS deficient | HCHO | 42 (21) |
| AfdS1-5 | None | afdRTS+ | None | 37 (15) |
| AfdS1-5 | None | afdRTS+ | MeOH | 410 (176) |
| AfdS1-5 | None | afdRTS+ | HCHO | 371 (37) |
| JWH1 | None | afdRTS and rfdRTS deficient | None | 19 (1) |
| JWH1 | None | afdRTS and rfdRTS deficient | MeOH | 19 (1) |
| JWH1 | None | afdRTS and rfdRTS deficient | HCHO | 20 (2) |
a
Standard deviations are in parentheses. Averages are data from activities of at least three independent isolates.
b
MeOH, 100 mM methanol was added to culture for 3 h prior to analysis; HCHO, 50 μM formaldehyde was added to culture for 1.5 h prior to analysis.