FIG. 2.

A. Organization of the phenol gene cluster of T. aromatica (GenBank accession number AJ272115). The numbers refer to the ORF numbers of T. aromatica. Arrows indicate the direction of transcription. Black, genes encoding subunits of phenylphosphate synthase; grey, genes encoding subunits of phenylphosphate carboxylase. Genes coding for phenol-induced proteins identified in two-dimensional gel electrophoresis are indicated by stars (16, 43). ORF11 is a putative σ⁵⁴-dependent regulator. ORFs 1 to 3 are subunits of phenylphosphate synthase. ORFs 4 to 6 and 12 are subunits of phenylphosphate carboxylase. ORFs 7 and 8 are putative ubiD/ubiX type (de)carboxylases (16, 43). ORFs 9, 10, 13, X, and 16 are hypothetical. ORF14 is similar to aromatic acid:CoA ligases. ORF15 is similar to proteins involved in the meta pathway of aerobic phenol metabolism in various species of bacteria. Black bars below the ORFs (b to o) represent the locations of fragments amplified in RT-PCR experiments. B. Results of RT-PCR experiments. a: negative control; fragment a could not be amplified on the RNA level; M: molecular mass standard; e: weakness of signal is due to suboptimal primer binding and is also observed in PCR experiments with chromosomal DNA. For details, see Materials and Methods.