Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Purification of ORF1 protein overproduced in E. coli TUNER. SDS-PAGE (10%) of various steps of the purification is shown. Lanes: 1, molecular mass standard; 2, total cell protein of induced E. coli cells (30 μg of protein); 3, soluble fraction of cell extract (induced cells) (20 μg of protein); 4, DEAE-Sepharose fraction (6 μg of protein); 5, Butyl-Sepharose fraction (5 μg of protein); 6, Mono-Q fraction (2 μg of protein).
