FIG. 5.

Characterization of phenylphosphate synthase. A. Study of ATP-dependent net phosphorylation of [¹⁴C]phenol to phenylphosphate (P) and of [¹⁴C]phenol exchange into phenylphosphate (E). From left to right: either ORF1 (0.2 mg) alone, ORF2 alone (0.2 mg), or ORF1 and ORF2 combined (0.2 mg each) were tested in the two assays (P and E). B. Study of the incorporation of ³²P into phenylphosphate from either γ-³²P-labeled ATP ([γ-³²P]) or β-³²P-labeled ATP ([β-³²P]). Autoradiograms of TLC plates are shown. For experimental details, see Materials and Methods.