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Purification of overproduced ORF1 from 10 g of cells (fresh cell mass) of E. coli TUNER (pET vector system)a
| Purification step | Total activity (nmol min−1) | Total protein (mg) | Sp ac (nmol min−1 mg−1) | Yield (%) | Enrich- ment (fold) |
|---|---|---|---|---|---|
| Soluble extract | 24 | 400 | 0.06 | 100 | 1 |
| DEAE-Sepharose | 27.5 | 50 | 0.55 | 114 | 9.2 |
| Butyl-Sepharose | 16.1 | 18.7 | 0.86 | 67 | 14.3 |
| Mono-Q | 10.5 | 9.5 | 1.1 | 43 | 18.3 |
The exchange of radioactivity from [14C]phenol into phenylphosphate was measured.
