TABLE 3.
Levels of induction of L. plantarum genes in vitro by 0.1% bile and in situ in the duodenum of a mouse model compared to the level in MRS without bile
| lp no. (gene) | Function | Fold induction ina:
|
|
|---|---|---|---|
| MRS + 0.1% bile | Duodenum | ||
| lp_0237 | Integral membrane protein | 23.7b | 12.5b |
| lp_0775 (argG) | Argininosuccinate synthase | 4.3b | 28.9b |
| lp_1027 (fusA2) | Elongation factor | NDc | 2.5 |
| lp_1898 (pfk) | 6-Phosphofructokinase | ND | 1.4 |
The coefficient of variation among replicates (n = 3) was <25%.
The relative expression level of the target gene is significantly different from the level observed in MRS lacking bile according to the pairwise fixed reallocation randomization test (P < 0.05).
ND, not determined.