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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Biomaterials. 2016 Jun 8;102:209–219. doi: 10.1016/j.biomaterials.2016.05.055

Figure 3. Activated neutrophils act synergistically with complement to damage MSCs.

Figure 3

MSCs (2×104) were labeled with BCECF and incubated for 30 min with 30% NHS alone (NHS) or 2×104 HL60 cell-derived neutrophils alone (Neut) or in the presence of various concentrations of wortmannin (0, 0.001, 0.01, 0.1, and 1 μM) (Neut + Wort), or 30% NHS plus 2×104 neutrophils alone (Neut + NHS) or in the presence of various concentrations of wortmannin (0, 0.001, 0.01, 0.1 and 1 μM) (Neut + NHS + Wort), then MSC damage was quantified using the BCECF release assay. The data are representative results in triplicate for 3 independent experiments and show the mean ± SD, One-way ANOVA and Tukey post-hoc tests were used for data analysis, *p<0.05