Figure 6. Painting factor H onto MSCs protects MSCs from the synergistic effect of complement and neutrophils.

(A) MSCs (2×10⁴) were incubated with 0, 2.5, 5, or 10 μg of EDC-activated CFH (Act. CFH) or 10 μg of non-activated CFH (Ctrl) for painting, then were labeled with BCECF, washed, then incubated with 30% NHS in GVB++ for 30 min and MSC damage was quantified using the BCECF leakage assay. Two-way ANOVA tests were used for data analysis, * p<0.05. (B) MSCs (2×10⁴) were painted with (CFH-MSC) or without (Ctrl-MSC) 10 μg of EDC-activated CFH, labeled with BCECF, and then were incubated for 30 min with 30% NHS alone (NHS), 2×10⁴ neutrophils alone (Neut.) or 30% NHS plus with 2×10⁴ neutrophils (NHS+Neut), when MSC damage was measured using the BCECF leakage assay. The data are representative of results in triplicate for 4 independent experiments and show the mean ± SD, One-way ANOVA and Tukey post-hoc tests were used for data analysis, *p<0.05