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. Author manuscript; available in PMC: 2017 Feb 3.
Published in final edited form as: Anal Chem. 2016 Nov 15;88(23):11584–11592. doi: 10.1021/acs.analchem.6b02998

Figure 4. Calculation of the motif prediction scores for transferrin.

Figure 4

A) Each motif score for a particular lectin was multiplied by the fluorescence signal for that lectin. The resulting products were summed over all lectins for each motif, giving the motif prediction scores for the unmodified glycans. The calculation was the same for the glycans after modification by sialidase 1 (panel B) and sialidase 2 (panel C), using the Δmotif scores and the Δintensities. The final step was to sum the motif prediction scores over all conditions. D) The top motif was 6-sialyl N-acetyl-lactosamine connected to unbranched mannose. Some of the top 5 motifs had high scores only after summing over the enzyme conditions.