FIG. 2.

Schematic representation of plasmids used in scanning mutagenesis. The c2 ori fragments carrying deletions in the transcript-encoding sequence were cloned into pVA891 and assayed for the ability to replicate in L. lactis. The numbers above the lines indicate the sizes (in base pairs) of the fragments 5′ and 3′ of the deletion. The spaces between the solid lines indicate the positions of deletions, and the numbers preceded by Δ indicate the lengths of the deletions (in base pairs).