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Impeded RsaA transport in rsaF mutant (JS1009) and RsaA mutant (JS1003 Hps12furin). Whole-cell preparations were loaded on SDS-12% PAGE gels and compared by Western blotting with anti-188/784 RsaA antibodies. The NA1000 strain was subjected to low-pH extraction before whole-cell preparations were made to minimize the levels of S-layer. No RsaA breakdown products are present in the mutants. Lanes: 1, NA1000; 2, JS1009; 3, JS1003::pWB9:723/furin; 4, JS1003.
