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Colloidal gold labeling of surface-displayed RsaF. Surface-displayed RsaF (A and B) was determined for the JS1001 strains overexpressing rsaFa or rsaFb (data not shown) by electron microscopy with anti-RsaFa and colloidal gold labeling. (A) JS1001 shows wild-type levels of RsaF with moderate labeling. (B) The JS1001 rsaFa strain shows a significant increase in surface display of RsaF. Note that only about 20% of the JS1001 rsaFa cells showed a significant increase in RsaF display.
