Figure 3. VACV boost increased the cytolytic activity of antigen-specific CD8⁺ T cells.

Four weeks after immunization, splenocytes from naïve mice were coated with OVA peptides or left untreated. Peptide-coated cells and non-coated cells were respectively stained with high or low concentration of CFSE, mixed and adoptively transferred into the immunized mice. (A) Representative histograms of CFSE-transferred cells in the spleen 4 hr after injection, percentage specific killing of OVA peptide pulsed cells in the blood (B) and spleen (C).