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. 2017 Feb 3;7:41378. doi: 10.1038/srep41378

Figure 2. The degraded polyQ tract in M. minutoides SRY does not compromise protein stability.

Figure 2

(a) Schematic of EGFP-tagged matSRY and minSRY proteins (g-matSRY and g-minSRY, respectively). EGFP-tagged musSRY (g-musSRY) and musSRY lacking the polyQ domain (g-musSRYΔQ) are also shown here for comparison. (b) Both g-minSRY and g-matSRY were detected in 15P-1 stable cell lines by Western blotting using an anti–EGFP antibody. g-musSRY and g-musSRYΔQ were included as positive and negative controls, respectively. Predicted molecular weight: g-musSRY, 77.1 kDa; g-musSRY ΔQ, 47.9 kDa; g-minSRY, 50.7 kDa; g-matSRY, 59.9 kDa. A blot using anti–α-Tubulin served as loading control. Full-length blots are presented in Supplementary Fig. S15. (c) Both g-matSRY and g-minSRY were detected in the nuclei of 15P-1 cells by immunofluorescence using an anti–EGFP antibody. g-musSRY and g-musSRYΔQ were included as positive and negative controls, respectively.