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. 2017 Feb 3;7:41378. doi: 10.1038/srep41378

Figure 3. M. minutoides SRY fails to activate the M. musculus TESCO reporter due to the degeneration of its C-terminal polyQ tract.

Figure 3

(a) minSRY, unlike musSRY and matSRY, failed to synergize with musSF1 to activate a luciferase construct containing M. musculus TESCO (compare green arrows). (b) Similar to minSRY, the musHBminQ mutant failed to activate musTESCO-Luc in the presence of musSF1 (compare orange arrows), whereas the mutant minHBmusQ restored the transactivation ability to that of musSRY (compare blue arrows). The luciferase activity of musTESCO co-transfected with the empty vector in the absence of SF1 was set to 1. SRY constructs do not activate TESCO in the absence of SF1, and thus the −SF1 data essentially showed unchanged base level activities of the musTESCO-Luc reporter. Therefore, for simplicity, only the + SF1 data are presented here, as mean ± s.e.m (n = 3). Dashed lines indicate the level of baseline (empty vector + SF1). (**) P < 0.01 vs. empty vector, or as indicated, one-way repeated measures ANOVA with Holm-Sidak multiple comparisons test. ns, not significant.