TABLE 2.

MICs of metal ions for W. metallidurans strains with mutations in genes for cation diffusion facilitators^a

Strain	Relevant genotype	Plasmid(s)	MIC of metal salt (μM)b
			CoCl2	NiCl2	ZnCl2	CdCl2
AE104			400	400	200	250
AE104	dmeF::pLO2		4	300	200	100
AE104	fieF::pLO2		300	400	200	200
CH34		pMOL30, pMOL28	6,000	2,500	4,500	1,500
CH34	dmeF::pLO2	pMOL30, pMOL28	50	2,500	4,500	1,500
CH34	fieF::pLO2	pMOL30, pMOL28	50	2,500	4,500	1,500
AE128		pMOL30	4,500	ND	ND	ND
DN182	ΔczcD	pMOL30-4	2,500	ND	ND	ND
AE128	dmeF::pLO2	pMOL30	15	ND	ND	ND
AE128	dmeF::pLO2 ΔczcD	pMOL30-4	4	ND	ND	ND

^aStrains were grown for 48 h in minimal medium, diluted 1:100, and streaked on minimal agar plates with increasing metal concentrations. Growth was monitored for 5 days at 30°C. The results were confirmed by at least three independent experiments.

^bND, not done.