Genes with no transposon (Tn) insertions are enriched in some functional classes but not in others. The percentage of genes with no transposon insertions in each functional class is indicated. Abbreviations: Biosyn/Metab, biosynthesis and metabolism; Cell Div, cell division; Cell Env, cell envelope; Chap, chaperone; Comp, DNA competence; Detox, detoxification; DNA/RNA, DNA and RNA metabolism; Mot, motility; Out Mem, outer membrane; PAI, pathogenicity island; Prot Syn, protein synthesis; Reg, regulation; Res Mod, restriction modification; Trans, transporters; Unknown Hyp, unknown hypothetical.