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. 2004 Dec;186(23):7971–7979. doi: 10.1128/JB.186.23.7971-7979.2004

FIG. 3.

FIG. 3.

Northern analysis of the ilv-leu transcript. RNA samples prepared from cells of strains 168 (wild type) (lane 1), FU459 (tnrA::cat) (lane 2), BFS2411 (ysnD::pMUTIN) (lane 3), and FU657 (tnrA::cat ysnD::pMUTIN) (lane 4), which had been grown in MM medium containing 13.6 mM sodium glutamate instead of glutamine to an optical density at 600 nm of 0.6, were subjected to Northern analysis with an ilv-leu leader-specific 32P-labeled DNA probe (l-ilvB) (Fig. 1). Each lane contained 10 μg of total RNA. The positions of size markers are indicated on the left. The arrow indicates the position of the 0.4-kb transcript terminating before ilvB.