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. 2016 Jan 11;17(2):167–177. doi: 10.15252/embr.201540789

Figure 1. Tie2‐Cre‐mediated ablation of NgBR in endothelial cells impairs extraembryonic vascular development.

Figure 1

  • A
    Gross morphology of control and NgBRECKO embryos at E8.5, E9.5, and E10.5. Scale bar, 500 μm.
  • B
    Gross morphology of whole yolk sac at E9.5 and CD31 staining on yolk sac at E9.5 and E10.5 in NgBRECKO and control embryos. The control yolk sacs exhibited large vitelline vessels indicated with red and white arrowheads. Scale bar, 500 μm for whole‐mount yolk sac and 100 μm for CD31 staining.
  • C–F
    Hematoxylin and eosin stained sections of E9.5 placenta of control and NgBRECKO embryos. Labyrinthine layer of mutants is markedly thinner compared to that of the control. Magnification of the boxes in (C, E) is shown in (D, F), respectively. Embryonic vessels and maternal vessels are marked with blue and black lines, respectively. gc, giant trophoblast cell layer; la, labyrinthine layer. Scale bar, 200 μm.