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. 2016 Jan 11;17(2):167–177. doi: 10.15252/embr.201540789

Figure EV4. Nogo‐B is not required for NgBR functions in endothelial cells during embryogenesis.

Figure EV4

  1. Staining with anti‐CD31 on hindbrain of E12.5 embryos after tamoxifen injection at E8.5. Scale bar, 100 μm.
  2. Western blot analysis for protein glycosylation in MLEC. Nogo‐A/B KO MLEC were isolated from Nogo‐A/B KO animals. Expression of VEGFR2 and CD31 was detected. Knockout cells were confirmed by detection with anti‐NgBR and anti‐Nogo‐B antibodies. Hsp90 was used as a loading control.
  3. Microsomal cisPTase activity assay for Nogo‐A/B KO MEF. No significant difference was detected between control and NogoA/B KO cells. cisPTase activity assay was performed as described in 4. NS, not significant (P > 0.05). P‐value was calculated by unpaired Student's t‐test. Data are mean ± SEM, n = 3 per group.